What Is Lamin A Protein

Its initial form called prelamin a undergoes a complex series of steps that are necessary for the protein to be inserted into the lamina.

What is lamin a protein.

The lamins are the major architectural proteins of the animal cell nucleus. The lamin a protein must be processed within the cell before becoming part of the lamina. Lamin a consists of a central rod domain flanked by hydrophobic n and c terminal domains. Depolymerization of the nuclear lamins leads to disintegration of the nuclear envelope.

This protein plays an important role in determining the shape of the nucleus within cells. In this case lamin a may be dialyzed into tris hepes or napi buffers with ph 8 0 8 5 and containing 250 mm nacl and 1 mm dtt in which most of the lamin protein should stay soluble at concentrations below 50 μg ml for several hours. Lamins line the inside of the nuclear membrane where they provide a platform for the binding of proteins and chromatin and confer mechanical stability. They have been implicated in a wide range of nuclear functions including higher order genome organization chromatin regulation transcription dna replication and.

Lamin c does not have to undergo this processing before becoming part of the lamina. Hutchinson gilford progeria syndrome hgps omim 176670 is a rare disorder characterized by accelerated aging and early death frequently from stroke or coronary artery disease. Laminin interestingly is in the shape of a cross see here in fact a quick internet search turns up multiple websites selling t shirts mousepads stickers coffee mugs and a host of other items with a picture of the laminin protein. Mutations in lamin genes can result in several genetic laminopathies which may be life threatening.

Lamin a is targeted to the nuclear membrane by an isoprenyl group but it is cleaved shortly after arriving at the membrane. At ph and salt concentrations below this or at higher protein concentrations lamin a will tend to oligomerize. Lamin proteins are involved in the disassembling and reforming of the nuclear envelope during mitosis the positioning of nuclear pores and programmed cell death. Lamin a is a 664 amino acid protein with a molecular weight of 70 kda calculated 74 139 da and a pi of 7 0 theoretical pi 7 4.

It stays associated with the membrane through protein protein interactions of itself and other membrane associated proteins such as lap1. The lamin a protein must be processed within the cell before becoming part of the lamina. After farnesylation at the caax box motif. Mutations in the lmna gene cause hutchinson gilford progeria syndrome the lmna gene provides instructions for making a protein called lamin a.

Over the past few months we have received numerous questions concerning a popular christian icon the protein laminin. Lamin c does not have to undergo this processing before becoming part of the lamina.